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D'Arcy Meyer-Dombard

Department of Earth, Atmospheric and Planetary Sciences
Massachusetts Institute of Technology
E34-546, 42-44 Carleton Street
Cambridge, MA 02142

phone 617.253.7850
fax 617.253.8630
email drmd@mit.edu

 

education

  • Ph..D. – Earth and Planetary Sciences, Washington University in St. Louis Oct., 2004
    Advisors: Prof. Jan P. Amend (Wash.U.)
    Thesis: Geochemical Constraints on Microbial Diversity of Hydrothermal Ecosystems in Yellowstone National Park
  • M.A. - Earth and Planetary Sciences, Washington University in St. Louis May, 2000
    Advisors: Prof. Everett L. Shock (now, ASU)
    Thesis: Investigating Thermophilic Habitats in Yellowstone National Park Using Geochemical Growth Media
  • B.S. – Environmental Studies, Washington University in St. Louis May, 1998
    Advisor: Prof. Robert E. Criss
    Thesis: d 18 O Variations in St. Louis , MO Precipitation with Climatic Variables

 

appointments
  • Postdoctoral Research Associate, Massachusetts Institute of Technology present
    Advisor: Roger Summons
  • Postdoctoral Research Associate, Washington University in St. Louis Dec. 2004 - Aug. 2005
    Advisor: Jan Amend

 

research interests
  • Geomicrobiology/Microbial Ecology
  • Ecosystem Evolution and Colonization Strategies of Thermophiles
  • Astrobiology
  • Geochemistry of Hydrothermal Systems

I approach geomicrobiology from an ecological perspective, using geochemistry, molecular, and microbiology in innovative ways to build ecological models of hydrothermal systems.

Microbial Diversity in Hydrothermal Environments:
I'm currently focusing on geomicrobial processes at interfaces between mildly reducing hydrothermal fluids and oxidizing surface sediments and waters in terrestrial systems (Yellowstone National Park) and shallow marine systems (Italy, Papua New Guinea). I'm interested in using molecular (genomic), chemical, and isotopic parameters to identify the metabolic strategies, nutrient/energy requirements, and geochemical signatures (lipid biomarkers) of thermophilic and chemolithotrophic Bacteria and Archaea.

In collaboration with E. Shock (ASU) and J. Raymond (LLNL), I am also pursuing a new initiative to assess correlations between metabolic genes and predicted energy sources. This pilot project, the first of its kind, follows a thermal/chemical gradient across the chemotrophic-phototrophic interface of a hotspring outflow channel, and will integrate metagenomics, geochemistry, and thermodynamic predictions of energy availability. Results will include functional profiles and phylogenetic analysis of orthologous environmental gene tags.

Thermophilic Communities in Arsenic-rich Thermal Systems of Papua New Guinea (PNG) :
I am continuing work with Jan Amend (Wash.U.) and Thomas Pichler (USF), detailing ecosystem response to elevated arsenic concentrations in shallow submarine hydrothermal systems in PNG (see http://www.biocomplexity.usf.edu/ ). This work investigates potential microbial influences on the adsorbtion of arsenic to Fe(III) oxyhydroxide precipitates surrounding the vents. Efforts focus on culturing thermophiles from these systems in growth media designed from the geochemical composition of the thermal fluids, targeting a variety of metabolic processes using arsenic and iron redox chemistry. Pure isolates will be screened for in situ activity of the arrA gene (arsenite respiration), and arsenate oxidizing isolates may help identify the genetic apparatus responsible for arsenate redox metabolisms. My 16S rRNA gene survey of sediments and biofilms surrounding the vent systems focuses on changes in community structure as a function of decreasing arsenic concentrations. These efforts may elucidate means of remediation for arsenic contamination in groundwater, a world-wide environmental concern.

 

research experience
  • Postdoctoral Research Associate, Massachusetts Institute of Technology Present
    * Ecophysiology of deeply-branching Bacteria and Archaea in hydrothermal environments:
    - Habitats, colonization and potential co-evolution of Aquificales and uncultured Crenarchaea in Yellowstone, Vulcano ( Italy ), and Ambitle ( Papua New Guinea )
    - Investigation of carbon sources of the above mentioned
    - Investigation of potential biomarkers for thermophilic Bacteria and Archaea
  • Postdoctoral Research Associate, Washington University in St. Louis 2004
    * Ecosystem diversity under elevated arsenic concentrations, Papua New Guinea:
    - Using geochemistry, molecular biology, microscopy and culturing techniques
    - Investigating thermophilic organisms living in shallow marine thermal vents containing extremely elevated concentrations of arsenic
    - Modeling ecosystem dynamics and stress response
  • Doctoral Candidate, Washington University in St. Louis 1998-2004
    * Building Earth analogs for the study of Astrobiology:
    - Terrestrial hydrothermal ecosystems in Yellowstone National Park
    - Shallow marine hydrothermal systems in Vulcano , Italy and Ambitle, Is. PNG
    * Ecosystem diversity in Yellowstone National Park hot spring environments:
    - Using molecular biology techniques and fluorescent microscopy
    - Comparison of thermophilic community diversity in three geothermal systems of variable geochemistry
    * Geochemistry of Yellowstone hot springs:
    - Collected geochemical data from an average of fifty hot spring features over four field seasons from 1999. These data comprise the largest, most continuous collection of geochemical data from these thermal features, allowing investigation of temporal and seasonal variability.
    * Culturing of thermophiles using geochemical data as templates for growth media:
    - Developed a method of designing aqueous growth media for culturing thermophiles specific to individual thermal features, using the above geochemical database.
    - Obtained pure isolates of novel thermophiles
    - I am currently working to identify and characterize 19 novel species from these cultures.
    * Calculations of energy available for microbial growth in geochemical systems:
    - Determined the theoretical energy available from microbially mediated geochemical reactions in the Fe, S, N, H redox systems for the hot springs in my study.

 

publications

•  Amend, J.P., Meyer-Dombard, D.R., Sheth, S.N., Zolotova, N., and Amend, A.C. (2003), Palaeococcus helgesonii , sp. nov., a facultatively anaerobic, hyperthermophilic Archaeon from a geothermal well on Vulcano Island , Italy . Archives of Microbiology , 179, 394-401.

•  Amend, J.P., Rogers , K.L., and Meyer-Dombard, D.R., (2004), Microbially mediated sulfur-redox: Energetics in marine hydrothermal vent systems. IN: Sulfur Biogeochemistry-Past and Present.GSA Special Paper 379 (Eds., Amend, J.P, Edwards, K.J., and Lyons, T.W.),p.17-34.

•  Shock, E.L. Holland, M. Meyer-Dombard, D. and Amend J.P. (2005, in press) Geochemical sources of energy for microbial metabolism in hydrothermal ecosystems: Obsidian Pool, Yellowstone National Park , USA . Geothermal Biology and Geochemistry in Yellowstone National Park . (W. Inskeep, T. McDermott, eds.), Thermal Biology Institute, Montana State University (pp. 95-112).

•  Meyer-Dombard, D.R., Shock, E.L., Amend, J.P. (2005), Archaeal and Bacterial Communities in Geochemically Diverse Hot Springs of Yellowstone National Park , USA . Geobiology , 3 , 211-227.

•  Meyer-Dombard, D.R., Shock, E.L., and Amend, J.P., Geochemical templates in growth media design: Trace element effects on culturing thermophiles. (submitted, Applied and Environmental Microbiology, in review).

•  Meyer-Dombard, D.R., M.R. Osburn, R. Price, T. Pichler, J.P. Amend. Prokaryotic populations in heated sediments of a shallow submarine hydrothermal system, Ambitle Island , Papua New Guinea . (submitted, FEMS Microbiology Ecology ).

•  Meyer-Dombard, D.R., Osburn, M.R., Shock, E.L., Amend, J.P. Novel thermophiles from Sylvan Spring, a slightly acidic sulfate-chloride hot spring in Yellowstone National Park : physiological characteristics and ecological implications. (in preparation for Archives of Microbiology ).

•  Shock, E.L., Meyer-Dombard, D.R., Amend, J.P., Osburn, G.R., and Fischer, T. Controls on the forms and abundances of geochemical energy in hot spring habitats, Yellowstone National Park , USA . (in preparation for Geobiology ).



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last updated: Tuesday 14 February, 2006 9:44